Conduct a final prep of the incision site, alternating between both 2% chlorhexidine and 70% isopropyl alcohol or 10% povidone-iodine and 70% isopropyl alcohol.Administer analgesics as per described in the AUP.If necessary to stabilize the animal, secure to the surgical surface with tape or light weights. Move animal to the surgical area placing the animal on a clean warm surface such as a sterile surgical drape.Second step of surgical scrub: The intended incision site will be prepped with two consecutive applications alternating between both 2% chlorhexidine and 70% isopropyl alcohol or 10% povidone-iodine and 70% isopropyl alcohol. Wipe off with alcohol or distilled water on a gauze Allow the soap to stay on site for five minutes. Manually scrub the surgical area for two minutes making sure to keep hair surrounding the shaved area to get wet. This is intended to remove all oils and proteins. First step of surgical scrub: Prepare the area by scrubbing the area using either 4% chlorhexidine solution or iodine based scrub such as Betadine + detergent.Remove any excess hair with a vacuum or tape.Shaved area should have large enough margins around where incision will be but not too large to cause excessive heat loss. Shave enough hair from the patient to minimize the chance that it can contaminate the incision site.Instruments are then placed on a sterile surface to cool prior to using on another animal (maximum five).Rinse surgical instruments with sterile saline and then be placed in the bead sterilizer as per manufacturer’s instructions.*This procedure can be followed for a maximum of five surgeries before a new sterile pack is required.*.If surgeries are conducted on multiple rodents and the lab does not have surgical packs available for each animal, the instruments may be cleaned with 70% isopropyl alcohol ensuring that all tissue, blood, and debris have been physically removed. Have enough surgical packs for each surgical procedure.Lay out instrument pack and remove first wrap so it is ready for the surgeon to access the inside sterile wrap.This can be achieved by steam, chemical (ethylene oxide -EtO) or dry (bead sterilizer) sterilizing methods. All surgical instruments should be sterilized initially.Surgery should be conducted in a separate area from where the animal was shaved and prepped.Surgical area should be disinfected prior to starting surgery.Procedures: Surgical space and instrument care:
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